Ovarian Microenvironment Modulation by Adipose-Mesenchymal Stem Cells and Photobiomodulation Can Alter Osteoblasts Functions In Vitro

Purpose In tissue engineering and regenerative medicine, stem cells are used to produce tissues and organs for both physiological and clinical applications. The interactions among different organs and systems are controlled by chemical and physical stimuli, such as those induced by mesenchymal stem cells (MSC) and photobiomodulation (PBM), respectively. The aim of the present study was to investigate the effects of adipose-derived MSC (AdMSC) and PBM modulations in adult female rat ovaries induced to polycystic ovary syndrome (PCOS), in terms of the number of bone marrow stromal cells obtained and the differentiation of these cells into osteoblasts during 14 days of in vitro culture. Methods Bone marrow cells were cultured for 14 days, in the absence and presence of testosterone, prior to analysis of different cellular parameters. The data were analyzed using ANOVA and Fisher’s tests, with statistically significant differences among the means considered for p