The activity of some Agomirs and Antigomirs as non small lung carcinoma suppressor

Background: non-small cell lung cancer (NSCLC) development, from tumor initiation to progression and metastasis play roles in cell proliferation, apoptosis, angiogenesis, epithelial-to-mesenchymal transition, migration, invasion, and metastatic colonization, as well as immunosuppression. Objective: To evaluate the agomirs, sense antagomir, and anti-sense sequences of the most effective ncRNA as a therapeutic tools via gene silencing, due to regression of A549 lung epithelial cell line cell line. Three non coding RNA molecules were transfected into cell lines, two of them were micro RNAs(mircroRNA135a and mircroRNA34a) agomir molecules and one was long non coding RNA(Adenovirus-HOX Antisense Intergenic RNA) sense molecules, in addition to other two molecules of antagomir of same microRNAs and one antisense molecules represented HOTAIR. Cell viability utilizing XTT (2,3-Bis-(2-Methoxy- 4-Nitro-5-Sulfophenyl)-2HTetra- zolium-5-Carboxan- ilide)was detected. Transfection efficiency of ncRNAs was determined by Real Time qPCR, Cell viability showed significant value with (mircroRNA135a and mircroRNA34a, HOTAIR) agomirs and sense molecule and antagomirs and antisense molecule of the same non coding RNA. WI38 cell line viability values were increased with all most agomirs and sense and sense transfection. Cell line images un- der inverted microscope showed a clear inhibition of cell line proliferation after transfection with antisense and antagomir molecule. Target gene expression inA549 cell line after molecule transfection showed insignifi-cant upregulation of TGF-ß and VEGF, TNF and SMAD while high up regulating of TP53, CASPASE8and IFNG with antagomirs and antisense molecule. The therapeutic mir34 index of transfec-tion elevated A549 cell line regression and consider as promising program for lung carcinoma treatment.