Insights into the antimicrobial properties of a cationic steroid and antibiofilm performance in PDMS-based coatings to potentially treat urinary infections

Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds. Cationic peptide antibiotics (CPAs) and ceragenins (CSAs) contain in their structures cationic groups and adopt a facially amphiphilic conformation, conferring t...

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Veröffentlicht in:Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2023-09, Vol.11 (36), p.8697-8716
Hauptverfasser: Neves, Ana Rita, Freitas-Silva, Joana, Durães, Fernando, Silva, Elisabete R, Rodrigues, Inês C, Mergulhão, Filipe, Gomes, Marisa, Teixeira-Santos, Rita, Bernardes André, Maria, Silva, Renata, Remião, Fernando, Pinto, Eugénia, da Costa, Paulo Martins, Sousa, Emília, Correia da Silva, Marta
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container_end_page 8716
container_issue 36
container_start_page 8697
container_title Journal of materials chemistry. B, Materials for biology and medicine
container_volume 11
creator Neves, Ana Rita
Freitas-Silva, Joana
Durães, Fernando
Silva, Elisabete R
Rodrigues, Inês C
Mergulhão, Filipe
Gomes, Marisa
Teixeira-Santos, Rita
Bernardes André, Maria
Silva, Renata
Remião, Fernando
Pinto, Eugénia
da Costa, Paulo Martins
Sousa, Emília
Correia da Silva, Marta
description Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds. Cationic peptide antibiotics (CPAs) and ceragenins (CSAs) contain in their structures cationic groups and adopt a facially amphiphilic conformation, conferring the ability to permeate the membranes of bacteria and fungi. Keeping these features in mind, an amine steroid, DOCA-NH 2 , was found to be active against reference strains and MDR isolates of Gram-positive Enterococcus faecalis and Staphylococcus aureus and Gram-negative Escherichia coli and Pseudomonas aeruginosa . The compound was active against all the tested microorganisms, having bactericidal and fungicidal activity, displaying minimal inhibitory concentrations (MICs) between 16 and 128 μg mL −1 . No synergy with clinically relevant antibacterial drugs was found. However, the compound was able to completely inhibit the biofilm formation of bacteria exposed to the MIC of the compound. For E. coli and E. faecalis , inhibition of biofilm formation occurred at half the MIC. Besides, DOCA-NH 2 inhibited the dimorphic transition of Candida albicans at concentrations 4 times lower than the MIC, and can reduce the microorganism virulence and biofilm formation was significantly reduced at both MIC and half the MIC. Polydimethylsiloxane-based coatings containing DOCA-NH 2 (0.5, 1.0, and 1.5 wt%) were prepared and tested against the E. coli biofilm formation under hydrodynamic conditions similar to those prevailing in ureteral stents. A biofilm reduction of approximately 80% was achieved when compared to the control. Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds.
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Cationic peptide antibiotics (CPAs) and ceragenins (CSAs) contain in their structures cationic groups and adopt a facially amphiphilic conformation, conferring the ability to permeate the membranes of bacteria and fungi. Keeping these features in mind, an amine steroid, DOCA-NH 2 , was found to be active against reference strains and MDR isolates of Gram-positive Enterococcus faecalis and Staphylococcus aureus and Gram-negative Escherichia coli and Pseudomonas aeruginosa . The compound was active against all the tested microorganisms, having bactericidal and fungicidal activity, displaying minimal inhibitory concentrations (MICs) between 16 and 128 μg mL −1 . No synergy with clinically relevant antibacterial drugs was found. However, the compound was able to completely inhibit the biofilm formation of bacteria exposed to the MIC of the compound. For E. coli and E. faecalis , inhibition of biofilm formation occurred at half the MIC. Besides, DOCA-NH 2 inhibited the dimorphic transition of Candida albicans at concentrations 4 times lower than the MIC, and can reduce the microorganism virulence and biofilm formation was significantly reduced at both MIC and half the MIC. Polydimethylsiloxane-based coatings containing DOCA-NH 2 (0.5, 1.0, and 1.5 wt%) were prepared and tested against the E. coli biofilm formation under hydrodynamic conditions similar to those prevailing in ureteral stents. A biofilm reduction of approximately 80% was achieved when compared to the control. 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B, Materials for biology and medicine</jtitle><date>2023-09-20</date><risdate>2023</risdate><volume>11</volume><issue>36</issue><spage>8697</spage><epage>8716</epage><pages>8697-8716</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds. Cationic peptide antibiotics (CPAs) and ceragenins (CSAs) contain in their structures cationic groups and adopt a facially amphiphilic conformation, conferring the ability to permeate the membranes of bacteria and fungi. Keeping these features in mind, an amine steroid, DOCA-NH 2 , was found to be active against reference strains and MDR isolates of Gram-positive Enterococcus faecalis and Staphylococcus aureus and Gram-negative Escherichia coli and Pseudomonas aeruginosa . 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source Royal Society Of Chemistry Journals 2008-
subjects Antibiotics
Antiinfectives and antibacterials
Bacteria
Biofilms
Cations
Coatings
Conformation
E coli
Fungicidal activity
Fungicides
Implants
Microorganisms
Minimum inhibitory concentration
Multidrug resistance
Peptide antibiotics
Polydimethylsiloxane
Steroids
Ureteral stents
Virulence
title Insights into the antimicrobial properties of a cationic steroid and antibiofilm performance in PDMS-based coatings to potentially treat urinary infections
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