Insights into the antimicrobial properties of a cationic steroid and antibiofilm performance in PDMS-based coatings to potentially treat urinary infections
Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds. Cationic peptide antibiotics (CPAs) and ceragenins (CSAs) contain in their structures cationic groups and adopt a facially amphiphilic conformation, conferring t...
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Veröffentlicht in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2023-09, Vol.11 (36), p.8697-8716 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds. Cationic peptide antibiotics (CPAs) and ceragenins (CSAs) contain in their structures cationic groups and adopt a facially amphiphilic conformation, conferring the ability to permeate the membranes of bacteria and fungi. Keeping these features in mind, an amine steroid,
DOCA-NH
2
, was found to be active against reference strains and MDR isolates of Gram-positive
Enterococcus faecalis
and
Staphylococcus aureus
and Gram-negative
Escherichia coli
and
Pseudomonas aeruginosa
. The compound was active against all the tested microorganisms, having bactericidal and fungicidal activity, displaying minimal inhibitory concentrations (MICs) between 16 and 128 μg mL
−1
. No synergy with clinically relevant antibacterial drugs was found. However, the compound was able to completely inhibit the biofilm formation of bacteria exposed to the MIC of the compound. For
E. coli
and
E. faecalis
, inhibition of biofilm formation occurred at half the MIC. Besides,
DOCA-NH
2
inhibited the dimorphic transition of
Candida albicans
at concentrations 4 times lower than the MIC, and can reduce the microorganism virulence and biofilm formation was significantly reduced at both MIC and half the MIC. Polydimethylsiloxane-based coatings containing
DOCA-NH
2
(0.5, 1.0, and 1.5 wt%) were prepared and tested against the
E. coli
biofilm formation under hydrodynamic conditions similar to those prevailing in ureteral stents. A biofilm reduction of approximately 80% was achieved when compared to the control.
Currently, multidrug-resistant (MDR) infections are one of the most important threats, driving the search for new antimicrobial compounds. |
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ISSN: | 2050-750X 2050-7518 |
DOI: | 10.1039/d3tb01185b |