DNA‐based fungal diversity in Madagascar and arrival of the ectomycorrhizal fungi to the island

Madagascar is known for its high diversity and endemism of fauna and flora. Fungi, however, have been largely overlooked in diversity and evolution studies on the island, and whether fungi exhibit the same patterns as animals and plants has yet to be further examined. We collected fungal sporocarps and ectomycorrhizal (EcM) roots during three opportunistic surveys in five forests in Madagascar and generated a dataset of fungal Internal Transcribed Spacer (ITS) DNA sequences. We analyzed them together with all publicly available fungal ITS DNA sequences and identified 620 Operational Taxonomic Units (OTUs) from Madagascar, 10% of which contained only sequences from our surveys. Two hundred and ninety‐two OTUs belonged to EcM species with /russula‐lactarius, /boletus, /tomentella‐telephora, /cortinarius, and /amanita being the most abundant EcM lineages. Overall, 60% of all fungi and 81% of the EcM species from Madagascar appear to be endemic. We conducted a phylogenetic analysis using all the OTUs in Amanitaceae, Boletaceae, and Russulaceae families to elucidate their relative timing of arrival in Madagascar. We found that most EcM species from Madagascar in the three families diverged less than 22 million years ago (mya), long after the separation of India and Madagascar (88 mya), which is consistent with a dispersal mediated process of arrival to the island. Our study provides the first comprehensive view of the overall DNA‐based fungal diversity in Madagascar and the current state of knowledge of EcM fungi based on DNA sequences, useful for further ecological and evolutionary studies. Abstract in Malagasy is available with online material. Madagasikara dia fantatra amin'ny habetsahan'ny fahasamihafana sy ny fisian'ny zavamaniry sy ny biby tokana an‐tany. Na izany aza dia tena mbola vitsy ny fikarohana momba ny olatra natao teto amin'ny nosy, ary tokony holalinina izany mba hahafantarana raha mitovy endrika amin'ny zavamaniry sy ny biby ny olatra. Nanangona “sporocarpe” na voan'ny olatra hita masosy fakan'ireo zavamaniry miainana miaraka amin'ny olatra na “ectomycorrhizal (EcM) roots” izahay nandritra ny fanadihadiana telo tamin'ny ala dimy teto Madagasikara. Izay nahafahana namoaka angona ADN “Internal Transcribed Spacer (ITS) sequences”. Natao ny famakafakana azy ireo niaraka tamin'ireo angona “ITS sequences” izay hita tamin'ireo fitehirizana “ITS sequences” natao hoan'ny rehetra. Araka ny vokatra azo dia 620 “Operational Taxonomic Units” (OTUs) tamin'i