СHONDROGENIC DIFFERENTIATION OF MSCS FROM VARIOUS SOURCES DURING CULTIVATION ON MATRIX FROM DECELLULARIZED PORCINE ARTICULAR CARTILAGE

Objectives: To compare the chondrogenic potential of mesenchymal stromal cells (MSCs) from various sources (adipose tissue (hADSC), dental pulp (hDPSCs), umbilical cord stroma (hUMSCs)) during their cultivation on a microdispersed matrix from decellularized porcine articular cartilage (MDC matrix). Methods: For decellularization sodium dodecyl sulfate, Triton X-100, ultrasound and DNase were used. 5×105 MSCs were cultivated in a chondrogenic medium for 21 days with 5 mg of MDC matrix dispersed in it, followed by a histological study of the morphology of the formed cell-engineered constructs (CECs). Results: During the cultivation of hDPSCs on the 21st day a single cell-matrix conglomerate was formed, fibroblast-like cells grew, multicellular clusters appeared, and there were practically no visible signs of extracellular matrix (ECM) production by the cells. The proliferation of hUMSCs and hADSC on the matrix surface was also accompanied by the association of cartilage microparticles into large aggregates but, as opposed to hDPSCs, multilayered cellular zones of cells were formed inside the aggregates, which actively synthesized the ECM. The highest production of glycosaminoglycans by hUMSCs and hADSC in the composition of the MDC matrix indicates the differentiation of cells in the chondrogenic direction. And only in CECs with hADSC by day 21 we had visualized the highest production of collagen with a positively staining for collagen type II. Conclusions: CECs based on MDC matrix and hADSC are the most promising for the formation of a tissue equivalent of cartilage.