OPTIMIZATION OF A SINGLE-LUNG TRANSPLANTATION MODEL ON A RAT MODEL

Objectives: To develop a method of orthotopic single-lung transplantation in an experiment on a rat model. Methods: In our study, we used Wistar rats. As a preservative, we used a solution of Celsior in a volume of 15 ml. The lung removal procedure was performed under inhalation anesthesia of 3.5% Isoflurane. After tracheal intubation by direct laryngoscopy, we underwent median sternotomy, the wound edges were separated by a retractor. We injected 500 IU of heparin solution into the cavity of the right ventricle and cannulated the pulmonary artery with an 18-gram catheter. We isolated the anatomical structures of the left lung to prepare for the application of cuffs for subsequent anastomosis. Lung transplantation: access was performed through a left-sided anterolateral thoracotomy in the 4th-5th intercostal space. The elements of the root of the left lung were separated in a blunt and sharp way. After that, an angular vascular clamp was applied with simultaneous clamping of all elements of the lung root. After applying the clamp, the lung was cut off in the pleural cavity. Results: The rat survival before the end of the experiment was 80%, 2 rats died on the first day after transplantation due to massive hemothorax. The Pa02/FiO2 index at selective sampling was 437 ± 25 mm Hg. Morphological examination of lung samples showed intact architectonics of the lung parenchyma in all cases. Microatelectatic zones were distributed heterogeneously and occurred on separate segment. Conclusions: The developed model of orthotopic transplantation is simple to perform, cuffs using excluded surgical complications associated with the imposition of an anastomosis in the classical way. The proposed technique for performing transplantation on a rat model has shown its effectiveness and reliability.