Lipopolysaccharide Neutralizing protein in Miso, Japanese Fermented Soybean Paste

Intestinal flora typically amount to 100 trillion cells comprising 400 bacteria species. Gram negative bacteria of one kind in the flora have lipopolysaccharides (LPS), which stimulate intestinal activity. Fatality rates from severe diseases caused by LPS are 30–50%. According to recent probiotic th...

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Veröffentlicht in:Annals of nutrition and metabolism 2023-08, Vol.79, p.217
Hauptverfasser: Kanauchi, Makoto, Syoji, Naoki
Format: Artikel
Sprache:eng
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Zusammenfassung:Intestinal flora typically amount to 100 trillion cells comprising 400 bacteria species. Gram negative bacteria of one kind in the flora have lipopolysaccharides (LPS), which stimulate intestinal activity. Fatality rates from severe diseases caused by LPS are 30–50%. According to recent probiotic theories of health, vigorous lactic acid bacteria in yogurt are eaten by many people to improve their intestinal environment. Moreover, some lactic acid bacteria reportedly have activity for neutralizing LPS from Escherichia coli. Actually, since ancient times, miso has been regarded as able to alleviate intestinal failure. Miso, a fermented paste made from soybeans, is used traditionally for seasoning of food. Since ancient times, it has been a dietary source of protein and nitrogen in Japan because of its high nutritional value and important health functions such as the estrogen-like activity of isoflavones, anti-oxidation, and angiotensin-converting-enzyme inhibition activity. Anti-inflammation effects of miso clearing mechanisms constitute a new healthy function. Today, similarly to yogurt, miso is regarded by many as a healthy food After some miso samples were analyzed, their LPS neutralization substrates were researched. First, their LPS neutralization activity was assayed. Consequently, only one miso sample has 85% elimination activity, 4 samples had 50–80% LPS neutralization activity, and 10 samples had 19–50% activity. The others failed to show activity. The one miso sample with the most neutralization activity among the samples also had the greatest amount of soluble nitrogen at 0.48%. Correlation analysis was conducted to assess LPS neutralization activity and other compounds in miso. The LPS neutralization activity was found to be related strongly with soluble nitrogen in miso; its coefficient R was 0.8329. A relation was found with fluorescent dye using ANS in miso. Therefore, LPS neutralization activity was found to have a relation with hydrophobic peptides in miso. Results indicate that miso neutralized LPS that might cause intestinal tract inflammation. After fractioning of miso, the LPS neutralization activity fractionations (proteins) were isolated from miso extract using Blue native polyacrylamide gel electrophoresis (PAGE). A protein found at 10–30 kDa on the polyacrylamide gel was identified using nano LC-MS/MS as 2S albumin in soybean (Glycine max). The protein had two LPS binding motifs: SKWQHQ (22 amino acid residues) and EKQKKKMEKE (131 am
ISSN:0250-6807
1421-9697
DOI:10.1159/000530786