Bioinformatic Analysis of the Transcriptome Profile of Adult Female Angiostrongylus cantonensis

ABSTRACT To perform transcriptional profiling of adult female Angiostrongylus cantonensis, three groups of adult female A. cantonensis were isolated and their mRNA was separated and sequenced through the next-generation sequencing (NGS) technology. Sequencing data were assembled into unigenes and transcripts. Finally, all unigenes and transcripts were annotated by querying the NR, Gene Ontology (GO), COG and KOG databases.A total of 71047 unigenes and 106652 transcripts were assembled. A. cantonensis was successfully annotated by the NR and GO databases, resulting in 8107 unigenes and 16105 transcripts. Meanwhile, 10422 unigenes and 20974 transcripts were annotated by the COG/KOG databases. ORF querying annotated 10531 unigenes and 28186 transcripts. It could be observed that the unigenes and transcripts associated with metabolism and parasitism of adult female A. cantonensis were highly expressed.In this study, the transcriptome of adult female Angiostrongylus cantonensis was analyzed and successfully annotated. Metabolism and parasitism related genes were highly expressed at adult female A. cantonensis.