Chlorogenic Acid Protects DNA against Double-strand Breaks: Evidence from Single Molecule Observation

Chlorogenic Acid Protects DNA against Double-strand Breaks: Evidence from Single Molecule Observation

A natural polyphenol chlorogenic acid (CGA) is regarded as an antioxidant by preventing oxidative damage. Among the oxidative damages, DNA double-strand breaks (DSBs) are considered the most severe. To study the protective effect of CGA against DSBs, we measured the time-dependent increase of DSBs c...

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Veröffentlicht in:Chemistry letters 2023-06, Vol.52 (6), p.524-527
Hauptverfasser: Ogawa, Haruto, Nishio, Takashi, Yoshikawa, Yuko, Sadakane, Koichiro, Yoshikawa, Kenichi
Format: Artikel
Sprache:eng ; jpn
Schlagworte:
Chlorogenic acid
Damage prevention
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Zusammenfassung:A natural polyphenol chlorogenic acid (CGA) is regarded as an antioxidant by preventing oxidative damage. Among the oxidative damages, DNA double-strand breaks (DSBs) are considered the most severe. To study the protective effect of CGA against DSBs, we measured the time-dependent increase of DSBs caused by photo-irradiation through a single molecule observation of DNA (166 kbp) using fluorescence microscopy. The results have been analyzed in relation to the two-step mechanism with single-strand breaks.
ISSN:0366-7022
1348-0715
DOI:10.1246/cl.230171