Covalent Organic Framework Based Crosslinked Porous Microcapsules for Enzymatic Catalysis

The design of porous microcapsules with selective mass transfer and mechanical robustness for enzyme encapsulation is highly desired for biocatalysis, yet the construction remains challenging. Herein, we report the facile fabrication of porous microcapsules by assembling covalent organic framework (COF) spheres at the interfaces of emulsion droplets followed by interparticle crosslinking. The COF microcapsules could offer an enclosed aqueous environment for enzymes, with size‐selective porous shells that allow for the fast diffusion of substrates and products while excluding larger molecules such as protease. Crosslinking of COF spheres not only enhances the structural stability of capsules but also imparts enrichment effects. The enzymes encased in the COF microcapsules show enhanced activity and durability in organic media as verified in both batch reaction and continuous‐flow reaction. The COF microcapsules offer a promising platform for the encapsulation of biomacromolecules. Porous microcapsules of covalent organic frameworks (COFs) were constructed for enzymatic catalysis, featuring bio‐friendly aqueous microenvironments and size‐selective porous shells that allow for the diffusion of substrates and products while excluding protease. The COF microcapsules containing Candida Antarctica lipase B (CALB) exhibited enhanced catalytic activity, recyclability, and durability for both batch and continuous‐flow reactions.