CHARACTERISATION AND BIOLOGICAL ACTIVITIES OF A FOREST Streptomyces T002 ISOLATED FROM WESTERN ALGERIA PRODUCING BLANCHAQUINONE

Exploring natural and uncharted ecosystems can yield novel biocontrol agents with potential benefits in both pharmaceuticals and agriculture. Microorganisms, being economically sustainable and biologically valuable, are a valuable source for the production of biocontrol compounds. The objective of this study is to assess the bioactive capabilities, specifically the antimicrobial and antioxidant properties, of Streptomyces sp. T002, which was isolated, for the first time, from the "Tifrit forest" in western Algeria. This study involved collecting 42 soil samples from Tifrit forest located in Western Algeria to evaluate their potential for producing bioactive substances. The T002 isolate, which demonstrated the highest level of bioactivity, was identified using various techniques such as morphological, physiological, biochemical, and molecular characterization. The bioactivity of the metabolites produced by this isolate was then assessed through in vitro evaluation. Finally, the crude extract was analyzed using liquid chromatography and mass spectrometry (LC-MS). The potent antimicrobial activity and significant antioxidant scavenging properties of the ethyl acetate extract of T002 were evident. The HPLC-MS analysis identified Blanchaquinone, a major compound in the extract, which may be responsible for its antimicrobial and antioxidant properties. The taxonomie position of T002 was determined to be similar to Streptomyces galilaus through the analysis of its 16S rRNA sequence. The strain T002 seems to hold great potential for producing new molecules that could be useful in the field of medicine.