Genetic diversity and population structure analysis of 378 introduced sweetpotato germplasm collections

Sweetpotato is originated from South and Central America, and the introduction of foreign species can not only increase the total quantity amount of germplasm resources of China, but also dramatically change the breeding bottleneck of sweetpotato with narrow genetic base. In this study, 378 sweetpotato germplasm collections from the national sweetpotato in vitro Genebank in Xuzhou were genotyped using 30 pairs of simple sequence repeat（SSR） primers by high-throughput nucleic acid fragment analyzer. Phylogenetic affinities among the introduced sweetpotato germplasm were analyzed to provide a theoretical basis for sweetpotato germplasm innovation and utilization. The bands were read using PROSize 3.0 software to establish the 01database. The 01 database were imported into Darwin software to calculate Jaccard genetic distance matrix, clustered analysis of genetic distance matrix using MEGA 10, and then annotated with iTOL. Population structure of sweetpotato germplasm was constructed using Structure 2.3.4. The r