GC-MS Analysis of Bioactive compounds present in different extracts of an Endemic tree Huberantha senjiana (Annonaceae) leaves

Objective: To explore the volatile chemical constituents present in different crude extracts of Huberantha senjiana (H. senjiana) leaves. Method: The coarsely powdered foliar parts of the leaves were extracted sequentially with solvents of increasing polarity like n-hexane, chloroform, ethyl acetate, isopropyl alcohol, and methanol. The extracts obtained were subjected to GC-MS analysis. Results: The analysis revealed the presence of different non-polar compounds in all five extracts in different concentrations. The bioactive Phyto compounds were recognized and characterized based on their retention and elution order in an analytical column. The mass spectra are matched with the inbuilt database of the NIST 8 library to identify the compound present. Conclusion: The present study is considered to be the preliminary study that reveals the presence of volatile components in different leaf extracts of H. senjiana which will serve as a reference for future studies.