Synthesis and Biomedical Activity of Aluminium Oxide Nanoparticles by Laser Ablation Technique

Aluminium oxide (Al2O3) nanoparticles (NPs) were formed via laser ablation of an aluminium target in deionised water (DIW) (Nd: YAG laser; wavelength: 1,064nm; different laser energies: 500, 800 and 1000 mJ; 30min). The optical, structural and morphological features of these Al2O3 NPs were investigated via ultraviolet/visible (UV/Vis) spectroscopy, scanning electron microscopy; X-ray diffraction (XRD) analysis, transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. Show that the average size of nanoparticles was between (21-48nm). The antibacterial activities of Al2O3 NPs were produced by utilising the well diffusion method against two pathogens (Pseudomonas aeruginosa and Bacillus cereus). Al2O3 NPs demonstrated significant antibacterial activity against P. aeruginosa and B. cereus compared with the control (P≤0.05). Al2O3 NPs had the best energy at 1000 mJ, indicating that they were more effective towards Gram +ve than Gram -ve bacteria. The synergistic/antibacterial activity of Al2O3 NPs exhibited potential antibacterial activity against the investigated species after being combined with imipenem and gentamicin, which had higher antibacterial action than Al2O3 NPs alone. Furthermore, as determined by DPPH, results suggested that Al2O3 NPs have antioxidant properties. Finally, Al2O3 NPs were tested for cytotoxicity against the breast cancer cell line (MCF-7), where 500mJ was 62.33±2.33, 800 mJ was 73.00±2.082 and 1000mJ was 85.00 ±1.732. The last was more effective than 500 mJ and 800 mJ and more efficient in penetrating cell membrane.