Chemiluminescence of horseradish peroxidase in water–ionic liquid microemulsion: an approach from catalytic point of view

Microemulsions of ionic liquids (ILs) are added to enhance the activity of peroxidase. From a catalytic standpoint, we provide the first research of the chemiluminescence (CL) features of luminol microencapsulated with horseradish peroxidase (HRP) in water–ionic liquid (W/IL) microemulsions. Water/AOT (sodium bis (2-ethyl-1-hexyl) sulfosuccinate)/hydrophobic IL [C8mim] [PF6] (1-octyl-3-methylimidazolium hexafluorophosphate)/1-hexanol constituted the system. Experiments demonstrated that the CL kinetic parameters, such as pseudo-first-order rise and fall rate constants for the chemiluminescence burst, CL intensity at time, maximum level intensity, total light yield, the intensity values at maximum CL, and time to reach maximum intensity, are dramatically affected as a function of W 0 (water/surfactant), co-surfactant percentage, pH, and reactant concentrations. At W 0 = 6, the maximum performance was achieved. The system exhibits more chemiluminescence properties than an enzymatic aqueous CL system and may be promoted without the need of an enhancer. Graphical abstract