Magnetic delivery and ultrasound-responsive release of chelating microcapsules for selective removal of urolithiasis

A novel urolithiasis treatment in which a chelating solution encapsulated in poly(lactic- co -glycolic acid); PLGA-based microcapsules was delivered magnetically to specific urolithiasis sites and then subjected to ultrasound (US) to release the chelating solution and dissolve the stones. Using a double-droplet microfluidics method, a hexametaphosphate (HMP) chelating solution was encapsulated in an Fe 3 O 4 nanoparticle (Fe 3 O 4 NP)-loaded PLGA polymer shell with a thickness of 90%), and an effective removal efficacy (>95%, 7 repeat cycles) of artificial calcium oxalate (5 mm in size) via a chelating effect. Eventually, the potential removal of urolithiasis in the body was verified using a PDMS-based kidney urinary flow-imitating chip with a human kidney stone (CaOx 100%, 5-7 mm in size) located in the minor calyx under an artificial urine counter flow (0.5 mL min −1 ). In the end, more than 50% of the stone, even in surgically tricky regions, was removed by 10 repeated treatments. Therefore, the selective approach of stone-dissolution capsules will help to develop alternative urolithiasis treatments to conventional surgical and systemic dissolution approaches. A novel strategy of urolithiasis treatment by magnetically delivering chelator encapsulated microcapsules to urolithiasis, followed by responsive release.