Evaluation of Thermostable Biomolecule Cocktail from Algal-associated Hot Water Spring Bacteria for Antibiofilm Activities

Controlling bacterial biofilms is a major target for industrial processes and thus is a tedious task under study in many research proceedings. The algal associated bacterial isolates from Unai Mata hot water spring, Gujarat, India, were screened for production of potent amylases, proteases and biosurfactants. The partially purified biomolecules were checked for effects of substrate concentration, cations and stability at extreme physical conditions like temperature (50 ℃) and acidic pH (5 and 6). Metal ions namely—Cu and Zn for amylase, while Zn and Na for protease, were found to be yielding least and highest activities, respectively. Best three isolates were selected for potential biomolecule production and sequenced for 16 s rDNA gene. The three isolates were found to be Stenotrophomonas sp. strain T1UM1, Pantoea sp. strain T1UM4 and Bacillus sp. strain T1UM8 with GenBank accession number MH764436, MH764437 and MH764438, respectively. The partially purified biomolecules cocktail showed effective antibiofilm activities at concentration of 1, 2, 5 and 10 U/ml or mg/ml against various bacteria tested at 1, 2 and 5 h treatments. The effects were different on the type of bacterium containing a combination of specific biomolecules. Thus, biomolecule cocktail reported here from hot water spring isolates has antibiofilm application potential in industries.