Antioxidant potential and high performance thin layer chromatography profiling of Siddha formulation Veppampoo mathirai

The present study was aimed to study the antioxidant potential and basic TLC, HPTLC fingerprinting profile of poly herbal Siddha formulation Veppampoo Mathirai used for hypertension to regulate blood pressure in siddha system of medicine.The antioxidant action was measured by ABTS free radical scavenging assay, lipid peroxidation and nitric oxide scavenging measure.The common modern techniques that help to evaluate the quality control of complex herbal medicine are Thin layer chromatography (TLC) and High performance thin layer chromatography (HPTLC). World health organization also indicated the above as a valuable tool to ensure the quality of medicinal plant products. Analysis of TLC was done by silica gel 60F254, 7X6 cm (Merck) and HPTLC by CAMAG Twin trough chambers. CAMAG software was used.The study revealed presence of prominent peak at 366nm in TLC analysis andtwelve prominent peaks in HPTLC analysis with Retention factor (Rf) value ranges from 0.02 to 0.86.ABTS scavenging assay, lipid peroxidation and nitric oxide scavenging showed EC50 values of aqueous extract of Veppampoo mathirai at55.32μg/ml, 51.3μg/ml and 51.31 μg/ml, separately. Theabove results revealed that aqueous extract of Veppampoo mathirai have solid antioxidant possibilities and also a good source of natural antioxidants