19G1-30 kDa proteins in the membrane of the pupal fat body of Bombyx mori interact with a hemolymph chymotrypsin inhibitor
In the silkworm, Bombyx mori, there are 30 kDa proteins that are expressed in the fat body and secreted into the hemolymph. A 19G1-30 kDa protein in the membrane fraction of the late larval midgut is a probable receptor for the hemolymph chymotrypsin inhibitor CI-8. Take-up of CI-8 into the pupal pe...
Gespeichert in:
Veröffentlicht in: | Journal of Insect Biotechnology and Sericology 2022, Vol.91(3), pp.3_033-3_039 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | In the silkworm, Bombyx mori, there are 30 kDa proteins that are expressed in the fat body and secreted into the hemolymph. A 19G1-30 kDa protein in the membrane fraction of the late larval midgut is a probable receptor for the hemolymph chymotrypsin inhibitor CI-8. Take-up of CI-8 into the pupal perivisceral fat body suggests that the 19G1-30 kDa proteins function as a receptor in the fat body. We used biochemical assays to investigate whether the 19G1-30 kDa proteins were the receptor for CI-8 in the pupal fat body. The results of affinity purification, far western blotting, and western blotting suggested that the 19G1-30 kDa proteins are the interacting factors of the membrane fraction of the pupal fat body. Tissue surface analysis indicated that these proteins were the canonical tissue-surface proteins. Immunological analysis showed that, after the take-up of CI-8 into the fat body, dissociation of the 19G1-30 kDa protein and CI-8 occurred followed by aggregation of the 19G1-30 kDa proteins to form protein granules. The results showed that the 19G1-30 kDa protein is a receptor of CI-8. |
---|---|
ISSN: | 1346-8073 1884-7978 |
DOI: | 10.11416/jibs.91.3_033 |