Advances in Spheroids and Organoids on a Chip

Multicellular spheroids and organoids are promising in vitro 3D models in personalized medicine and drug screening. They replicate the structural and functional characteristics of human organs in vivo. Microfluidic technology and micro‐nano fabrication can fulfill the high requirement of the engineering approach in the growing research interest in spheroids and organoids. In this review, spheroids and organoids are comparatively introduced. Then it is illustrated how spheroids‐ and organoids‐on‐a‐chip technology facilitates their establishment, expansion, and application through spatial‐temporal control, mechanical cues modeling, high‐throughput analysis, co‐culture, multi‐tissue interactions, biosensing, and bioimaging integration. The potential opportunities and challenges in developing spheroids‐ and organoids‐on‐a‐chip technology are finally outlooked. Multicellular spheroids and organoids are promising in vitro models in personalized medicine and drug screening. This review summarizes state of the art in spheroid‐ and organoid‐on‐a‐chip technology, including the advances in spatial and temporal control, mechanical cue modeling, high‐throughput analysis, co‐culture and multi‐tissue interactions, biosensing, and bioimaging integration. It also presents relative perspectives and future research directions.