Potential of Trichoderma viridae secondary metabolites extracted with ethyl acetate in suppressing the growth of Colletotrichum gloesporoides in vitro

Trichoderma viridae has the potential to be developed to control pathogens infecting plant parts above the soil surface by utilizing its anti-microbial secondary metabolites. The purpose of this study was to obtain the best concentration of secondary metabolites of T. viridae extracted with ethyl acetate organic solvent (ethyl acetate extract) in suppressing the growth of C. gloeosporoides in vitro. The experiment was arranged in a completely randomized design (CRD) with five treatments and four replications. The treatment was the concentration of ethyl acetate extract, consisting of 10%, 1%, 0.1%, 0.01%, and control (sterile distilled water). The variables observed were colony area, colony diameter, the number of conidia, conidia germination, and the number of propagules. The data were statistically analyzed using analysis of variance and LSD test at a 5% significance level. The results showed that the 10% concentration was the best in suppressing the growth of C. gloeosporoides with lower colony growth, number of conidia, number of propagules, and germination compared to other treatments.