Imidazole Pretreatment of Oil Palm Empty Fruit Bunches for Ethanol and Succinic Acid Co-production by Saccharomyces cerevisiae and Pichia stipitis
Oil palm empty fruit bunches (OPEFB) were pretreated with imidazole aiming to reduce the biomass recalcitrance and to co-produce ethanol and succinic acid. For such as purpose, two different yeasts ( Saccharomyces cerevisiae and Pichia stipitis ) were evaluated and compared. Imidazole pretreatment o...
Gespeichert in:
Veröffentlicht in: | Bioenergy research 2023-06, Vol.16 (2), p.990-1000 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Oil palm empty fruit bunches (OPEFB) were pretreated with imidazole aiming to reduce the biomass recalcitrance and to co-produce ethanol and succinic acid. For such as purpose, two different yeasts (
Saccharomyces cerevisiae
and
Pichia stipitis
) were evaluated and compared. Imidazole pretreatment of OPEFB at 180 °C for 1 h achieved a removal of 81.5% of lignin and a solid recovery of 53.0%. Enzymatic hydrolysis (24 h) of imidazole-pretreated OPEFB achieved 96.2% and 98.8% of glucose and xylose yields, respectively. Fermentation of the enzymatic hydrolysate with
S. cerevisiae
resulted in higher ethanol titer (8.9 g/L) and productivity (0.75 g/L/h) with lower fermentation time (12 h) compared to the fermentation (48 h) using
P. stipitis
(6.3 g/L and 0.13 g/L/h). Additionally, 1.11 g/L and 1.83 g/L of succinic acid were produced by
S. cerevisiae
(12 h) and
P. stipitis
(48 h), respectively. From a biorefinery sight, a total of 132.5 kg of ethanol and 16.4 kg of succinic acid could be produced from a ton of raw OPEFB, employing
S. cerevisiae
in a 12-h fermentation of the enzymatic hydrolysate obtained from imidazole-pretreated OPEFB. The results showed great potential of imidazole-pretreated OPEFB for ethanol production using
S. cerevisiae
, leading to efficient lignocellulosic biomass valorization and minimization of the agro-industrial waste. |
---|---|
ISSN: | 1939-1234 1939-1242 |
DOI: | 10.1007/s12155-022-10512-3 |