Sea phytoplankton identification Isochrysis aff galbana, Spirulina sp., Thalassiosira Sp., and Nitzchia Sp. to cultivate in MSSIP-2 medium for biomass and carbohydrates production

This research is isolate and identify the sea phytoplanktons for biomass production and determination of carbohydrate content. Sea phytoplanktons was cultivated in medium with “MSSIP-2” nutrient to multiply seeds and cultivate stocks. In the mass media cultivation, isolation and identification of sea phytoplankton was done based on size and certain characteristics. The measurement of temperature, salinity and pH solution also done in the media. The biomass weight of the density of selected phytoplankton was done by gravimetric method and the carbohydrate content was analyzed by glucose determination with Luff Schoorl. The isolation results indicate six types of sea phytoplankton: Isochrysis aff galbana, Spirulina sp., Thalassiosira sp., and Nitzchia sp. Specific growth rate of phytoplankton cells density is highest 0.0321 cells/hour at Isochrysis aff galbana and the lowest 0.0277 cells/hour at Thalassiosira sp. The highest biomass content 0.34 g.L−1 is Isochrysis aff galbana and the lowest 0.27 g.L−1 is Nitzchia sp.. The carbohydrate content is also varied, the highest 33.98% is Isochrysis aff galbana and the lowest 28.16% is Thalassiosira sp.