Modeling and tuning of glucose oxidase‐catalase system incorporating dissolved oxygen concentration

BACKGROUND Gluconic acid production by a glucose oxidase (GOx) and catalase (CAT) system has been proposed. However, the bioprocess optimal design and operation are limited by the lack of kinetic models of GOx involving oxygen as substrate. Herein, the GOx‐CAT system is modeled considering a continuous oxygen supply at a bioreactor lab scale. Initially, experiments and modeling for parameter estimations of KLa and the individual enzymes were conducted. Then, experiments and modeling for the GOx‐CAT system were performed for final model tuning. Additionally, the model quality was evaluated, allowing for a deeper understanding of the system phenomenology. RESULTS From the oxygen transport model tuning, a highly accurate KLa estimation was obtained (R2>0.98 and confidence interval