A simplification of isolation process of pinostrobbin from Boesenbergia pandurata (Roxb) rhizome

This research is aimed to simplify the pinostrobin isolation process from Boesenbergia pandurata (Roxb) rhizome. Boesenbergia pandurata (Roxb) rhizome is a class of flavonoid compounds containing many properties that are good for the human body. The rhizome is anti-bacterial, anti-fungal, anti-inflammatory, antispasmodic, anti-tumor, antiviral, anti-aging, anti-obesity, and antioxidant, and may act as an analgesic, antiseptic, or insecticide. The isolation method used is recrystallization with hot n-hexane maceration process. The crystals obtained were recrystallized until white crystals are formed. The crystals were tested by TLC and FTIR. The result of TLC is n-hexane and ethyl acetate with a ratio of 9:1. It shows a purple spot on 254nm under a UV lamp with a 0.81 Rf value. Based on the results of TLC and FTIR tests, the recrystallized white crystals were pinostrobin.