Effects of platelet-rich plasma combined with collagen matrix on arthritis chondrocyte regeneration and nanoparticles on oxygen free radicals

This study was to explore the effects of dopamine melanin nanoparticles (D–M nanoparticles) and collagen matrix combined with platelet-rich plasma (PRP) on arthritis chondrocytes and oxygen free radicals. 230–250 g rats were randomly divided into three groups, group A was treated with PRP, group B was treated with PRP combined with a collagen matrix, and group C was treated with PRP combined with collagen matrix and D–M nanoparticles. Each group was established an osteoarthritis model. The rats were sacrificed after the 10th week to detect and observe the proliferation of chondrocytes, changes in cell morphology, survival, changes in cartilage-specific genes, and the degree of cartilage in the model animals. compared with group A, both group B and group C had obvious cartilage differentiation and the effect of group C was better than group B; the cell proliferation in group C was much better in the other two groups; and the expression of cartilage-specific matrix proteoglycans in group C was better in contrast to group B and group A. Such results indicated that PRP could more effectively promote cartilage cell regeneration and prevent cartilage differentiation based on nanoparticle administration. In the D–M nanoparticle group, the level of free radicals in the synovial fluid of rats was greatly reduced ( P