In vitro response of shoots multiplications of date palm Phoenix dactylifera L. Cv Shakar to Jasmonic acid application under salinity stress

The study was conducted in the Tissue Culture Laboratory of the Date Palm Research Center - Basra University. shoots adventitious were used resulting from tissue culture of the apical and lateral shoots of date palms cv. Shakar by indirect shoot multiplication technique in vitro, where the application of several levels of JA was tested to reduce the effect of stress caused Salt stress using a bilateral interaction between NaCl at levels (0, 50, 100, 200) mM L-1 and JA at concentrations (0, 25, 50, 75) μM L−1, these materials were added to the special culture media of multiplication shoots adventitious, the results showed that treatment with NaCl at level (100, 200) mM L-1 led to significant inhibition of growth in terms of biomass, water content, and content of chlorophyll and carotene pigments, all of which were greatly improved upon application of JA. Treatment with NaCl salt increased the enzymatic antioxidant activity by enhancing the enzyme activity (POD) and (CAT). The addition of JA to the culture media either alone or under salt stress conditions, significantly improved growth, biomass, water content, chlorophyll, and carotene pigments, increased enzymatic antioxidant metabolism, and regulation of proline synthesis.