Fluorescence Studies on Flavonoid Diglycosides and Corresponding Deglycosylated Monoglycosides

A fluorescence quantitative detection method suitable for high-throughput screening of rhamnosidase catalytic activity on naringin was established. Detection of prunin at λ ex /λ em = 360/450 nm was based on the fluorescence difference after heating under alkaline conditions. Detection of naringin at λ ex /λ em = 325/400 nm was based on the difference in fluorescence in phosphate buffer. The method is suitable for prescreening many samples. A similar method can detect the fluorescence of hesperidin and hesperitin-7-o-glucoside, which provides a reference for the rapid detection of diglycosides and corresponding deglycosylated monoglycosides.