Phenol Degradation Performance in Batch and Continuous Reactors with Immobilized Cells of Pseudomonas putida

Phenol is a highly persistent environmental pollutant and is toxic to living organisms. The main objective of this study is to observe the phenol degradation performance by free and immobilized Pseudomonas putida (P. putida) in batch and continuous reactors, respectively. Batch experiments were evaluated to determine the maximum specific growth rate, saturation constant, inhibition constant, and cell yield. These kinetic parameters were used as the input values for the continuous-flow immobilized cells model. The immobilized cells model was validated by experimental results obtained from an immobilized cells continuous reactor. The model-predicted and experimental results showed good agreement for phenol effluent concentration in the continuous mode. In the steady-state condition, high phenol removal was achieved under various hydraulic retention times. The corresponding removal of phenol ranged from 93.3 to 95.9%, while the hydraulic retention times were maintained at 3.1–10.5 h. Furthermore, polyvinyl alcohol-immobilized cells with nanoscale particles were also prepared. The polyvinyl alcohol-immobilized P. putida cells with nanoscale Fe3O4 enhanced the ability of phenol degradation. The experimental results revealed that immobilized cells with nano-Fe3O4 had the highest phenol degradation performance at a low salinity of 1%. However, the advantage of the addition of nano-Fe3O4 was insignificant for phenol degradation at a higher salinity of 5%. The approaches of the batch and continuous column tests were practical in the treatment of actual phenol-containing wastewater.