Medicinal mushroom extracts exert differential effects in vitro on the release of inflammatory mediators by stimulated peripheral blood mononuclear cells from healthy, older adults

Isolated PBMCs were cultured at a density of 2×106 cells/mL and treated separately with 1 and 10 mg/mL of extracts (water-ethanol, WE; water-ethanol with Kakadu Plum, KP; water-glycerin, WG; water-glycerin liposomal, WGL) from medicinal mushrooms Hericium coralloides (HC) and Trametes versicolor (TV) for 3 hours. Following treatment, PBMCs were stimulated, separately, with 1 MOI rhinovirus A1 (RVA1), 0.1 multiplicity of infection (MOI) influenza A/H1N1pdm09 (H1N1), 5 ng/mL lipopolysaccharide (LPS), or 100 μg/mL house dust mite (HDM). For RVA1, H1N1: interleukin (IL)-1β, IL-6, IL-8, IL-10, interferon (IFN)-α2, and IFN-γ.