A highly sensitive electrochemical biosensor via short-stranded DNA activating LAMP(H+) to regulate i-motif folds for signal transduction
Exploring loop-mediated isothermal amplification (LAMP)-based electrochemical assay for short-stranded DNA biomarkers is intriguing. Herein, a HIV-specific DNA segment (tDNA) was introduced to implement a variant LAMP reaction, where the generated by-products H+, vLAMP(H+), acted as signal transduce...
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Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2023-04, Vol.380, p.133354, Article 133354 |
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Sprache: | eng |
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Zusammenfassung: | Exploring loop-mediated isothermal amplification (LAMP)-based electrochemical assay for short-stranded DNA biomarkers is intriguing. Herein, a HIV-specific DNA segment (tDNA) was introduced to implement a variant LAMP reaction, where the generated by-products H+, vLAMP(H+), acted as signal transducers for developing a sensitive electrochemical biosensor. As proof-of-concept, the tDNA-recognizable complement was programmed in a template hairpin (TH) that was ended with a 3′-terminus phosphate group to avoid non-specific elongation. Upon TH simultaneously hybridizing with tDNA and a precursor hairpin (PH), the PH underwent a loop-mediated extension with Bst polymerase and dNTPs, forming a new stem-loop hairpin in another terminus. Then the elongated product containing a double stem-loop dumbbell-like DNA structure (dDDS) was displaced and released. Using dDDS as an initiator, the vLAMP reaction occurred via consecutively cycled strand displacement for self-primed DNA incorporation. The resulting tDNA-dependent vLAMP(H+) was introduced in the modified electrode surface to guide the pH-sensitive i-motif (iM) folding. Thus, a signaling hairpin labeled with electroactive ferrocene (Fc) was dehybridized and closed, thereby bringing Fc close to the electrode interface for increased current signal readout that was highly sensitive to tDNA down to 0.034 fM. Our electrochemical biosensor would be applicable for detecting various short-stranded DNA or miRNA.
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●A variant LAMP-integrated electrochemical biosensor was developed for detecting short-stranded DNAs.●A HIV-specific DNA segment of 21-nt induced loop-mediated extension of a precursor hairpin.●A double dumbbell-like structure (dDDS) was formed and acted as template initiator to guide vLAMP.●The generated vLAMP(H+) was implemented to switch i-motif folds for dependent signal transduction.●The electrochemical readout of redox tag was highly target-sensitive and specific, simple and rapid. |
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ISSN: | 0925-4005 1873-3077 |
DOI: | 10.1016/j.snb.2023.133354 |