Discovery proteomics reveals potential protein signature associated with malignant phenotype acquisition in pleomorphic adenoma

Objective To analyze the proteomic profile of salivary pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA) samples and correlate them with the malignant transformation of the PA. Materials and methods Thirty samples (10 PA, 16 CXPA, and 4 residual PA) were microdissected and submitt...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Oral diseases 2023-04, Vol.29 (3), p.1017-1027
Hauptverfasser:	de Lima‐Souza, Reydson Alcides, Scarini, João Figueira, Lavareze, Luccas, Emerick, Carolina, Crescencio, Lívia Ramalho, Domingues, Romênia Ramos, Paes Leme, Adriana Franco, Mariz, Bruno Augusto Linhares Almeida, Bastos, Débora Campanella, Machado, Renato Assis, Tincani, Alfio José, Del Negro, André, Chone, Carlos Takahiro, Kowalski, Luiz Paulo, Egal, Erika Said Abu, Altemani, Albina, Mariano, Fernanda Viviane
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoma Adenoma, Pleomorphic - genetics Adenoma, Pleomorphic - metabolism Adenoma, Pleomorphic - pathology Apolipoprotein A carcinogenesis carcinoma ex pleomorphic adenoma Chromatography, Liquid Haptoglobin Hemoglobin Humans Liquid chromatography mass spectrometry Mass spectroscopy Oral cancer Phenotypes pleomorphic adenoma Protein transport Proteins Proteomics salivary gland neoplasms Salivary Gland Neoplasms - pathology Statistical analysis Synaptonemal complex Tandem Mass Spectrometry Tumors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Objective To analyze the proteomic profile of salivary pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA) samples and correlate them with the malignant transformation of the PA. Materials and methods Thirty samples (10 PA, 16 CXPA, and 4 residual PA) were microdissected and submitted to liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). The proteomic data and protein identification were analyzed through LC‐MS/MS spectra using the MaxQuant software. Results The proteomic analysis identified and quantified a total of 240 proteins in which 135 were found in PA, residual PA, and CXPA. The shared proteins were divided into six subgroups, and the proteins that showed statistically significant differences (p > 0.05) and fold‐change > or
ISSN:	1354-523X 1601-0825
DOI:	10.1111/odi.14102