The phospholipase-induced degradation of phosphatidylnucleosides

Hydrolysis of phosphatidylnucleosides, 5′-(rac-1-hexadecyl-2-palmitoyl-sn-glycero-3-phosphoryl)-3′-azido-3′-deoxythymidine and -2′,3′-didehydro-3′-deoxythymidine, effected by phospholipases (PL) A2, C, and D was studied to reveal the metabolism of these derivatives. It was shown that PLA2 deacetylates the glycerol residue at position 2, PLC is inactive, and PLD hydrolyzes the phosphatidylnucleosides to give free nucleosides.