Enhanced healing of skin wounds in ischemic rabbits using chitosan/hyaluronan/edaravone composite membranes: effects of laponite, carbon and silver-plated carbon nanofiber fillers

We examined the free-radical scavenging capacity of edaravone using the standard spectroscopic methods. Further, the impact of wound dressings composed of chitosan/hyaluronan with the addition of H atom and/or electron donoring edaravone in the absence and presence of laponite, C-nanofibers or silver-plated C-nanofibers was assessed on the healing of skin lacerations in ischemic ears of rabbits. The skin wound healing was compared with the untreated animals and animals, in which the wound was protected by a bicomponent chitosan/hyaluronan membranes. The in vitro results showed that edaravone in a dose-dependent manner was both free electron and H atom donor. The results of in vivo experiments showed that the addition of edaravone into chitosan/hyaluronan resulted in faster healing of skin wounds in ischemic rabbits. As proved also by histological investigation, the incorporation of laponite, C-nanofibers or silver-plated C-nanofibers into the composite membranes had significantly beneficial effects on skin wound healing.