Expression pattern analysis and interaction protein screening of cassava MYB transcription factor MeMYB60

Myeloblastosis（MYB） transcription factors widely involve in a variety of physiological and biochemical processes in plants, and play important regulatory roles in response to abiotic stress in plant. Based on the expression pattern of MYB members in cassava cultivars, an R2R3-MYB transcription factor, namely MeMYB60 was screened and cloned. Gene expression characteristics showed that MeMYB60 was specifically expressed in leaves of cassava, and negatively regulated by drought stress and low temperature. Moreover, this gene was also responded to ABA treatment in leaves of cassava. Promoter activity analysis showed that MeMYB60 could be expressed in guard cells, indicating that the expression of this transcription factor gene may be related to stomatal movement regulation in cassava. MeMYB60 protein was predominately located in the nucleus and had transcriptional activation activity. Its transcriptional activation domain was in the range of 194th–343rd amino acid residues at the C-terminal of the protein. The cD