Comparative Assay of Phytase Activity in Yarrowia lipolytica Strains Transformed with the Neutrophilic Phytase Genome from Obesumbacterium proteus in Batch Cultivation

An analysis is given of the dynamics of the activity of the phytase strain Yarrowia lipolytica that was transformed with the phytase genome from Obesumbacterium proteus based on the pUVLT2 vector with the VDAC (Voltage Dependent Anion Channel) mitochondrial porin promoter and induced under stress conditions when growing on low-value plant substrates. In the case of cultivation both in flasks and in a fermenter, an increase in the activity of intracellular phytase was observed when sunflower cake was used as a source of phytates. During the growth of the Y. lipolytica Po1f transformant under alkaline conditions, the VDAC promoter was activated as part of the pUV3-Op genetic construct, which was accompanied by an increase in the activity of intracellular phytase. The enzyme retained a high level of activity in Y. lipolytica pUV3-Op transformant cells after high-temperature treatment of the biomass in a spray dryer, which makes its use in the composition of feed additives for farm animals more effective.