Changes in the Electrical Characteristics of Premotor Interneurons and Serotonin-Containing Modulator Snail Neurons upon Developing a Contextual Conditioned Reflex and Its Reconsolidation

Here, we show that the development of a conditioned defensive reflex against the context in the terrestrial snail is accompanied by a depolarization shift of the membrane potential and a decrease in the threshold of action potential generation of premotor interneurons LPa3 and RPa3. There were no further significant changes in the membrane potential of the premotor interneurons in snails after reminder (initiation of reconsolidation) with subsequent injection of either the protein synthesis inhibitor anisomycin or saline. The threshold of action potential generation of these neurons decreases after training and remains unchanged after a reminder (initiation of reconsolidation) with subsequent injection of both anisomycin and saline. No significant changes were found in the membrane and threshold potentials of serotonin-containing neurons of the pedal ganglion Pd4 and Pd2 both after training and after a reminder followed by injection of solutions.