Vigorous Osteoinductivity Observed in Crude Bone Morphogenetic Protein Stored for 25 Years after Extraction at Room Temperature

Bone morphogenetic protein (BMP) is the only cytokine that induces heterotopic new bone. BMP has been classified as a subfamily of the TGF superfamily. The most important and essential aspect for the clinical application of BMP is the replenishment and reinforcement of bone defects and fragile areas with new bone. However, it is difficult to ascertain long term clinical results. In this study, we examined the osteogenic potential in mouse femoral fascia of crude BMP extracted from bovine bone that had been stored at room temperature for 25 years. The results showed vigorous osteogenesis. This study demonstrates the long-term stability of crude BMP.