Protein Fractions of Natural Killer Cell Lysates Affect the Phenotype, Proliferation and Migration of Endothelial Cells in vitro

Each stage of angiogenesis depends on the nature of the communication between endothelial cells and the microenvironment, and on the complex balance between stimulators and inhibitors of this process. Natural killer cells secrete a variety of cytokines and chemokines that can influence the microenvironment. The aim of this study was to assess the effect of protein fractions of an NK-92 natural killer cell lysate on the phenotype, proliferation and migration of the EA.hy926 endothelial cell line in an in vitro experimental model. We have shown that CD54 (ICAM-1) receptor expression by EA.hy926 cells after co-cultivation with 10–50 kDa and 30–80 kDa fractions was elevated in comparison with non-activated cells. The relative number of EA.hy926 cells with the CD54+ phenotype also increased after co-cultivation with the above fractions. The other remaining fractions (