Deconvoluted DSC curves of intestinal muscle layer following warm and cold ischaemic injury

Increasingly evidence seems that from the biological samples—mainly the summation of DSC scans of the human blood plasma—can be separated into main and well-known components by deconvolution method. This fact alone could be an important advance in thermoanalytical research, but mostly, it has not been detected in soft tissues yet. The other main reason was that in our previous studies, the histological examinations did not show any significant abnormalities in the intestinal wall muscle layer. Thus, the aim of current research was to measure the small intestinal muscular tissue scans by deconvolution method following different long warm and cold ischaemia animal experiments. Retrospectively, DSC curves obtained from the thermoanalysis of intestinal tissue in animal experiments investigated by deconvolution mathematical methods. Different warm ischaemic insults caused mild decrease after 3 and 6 h in T m3 and T m4 transitions in the myosin assigned transition and actin filament transition. After cold storage, the separated 5 melting components appeared in similar order, but the decreases were lesser than after warm ischaemia. Meanwhile, the calorimetric enthalpy which is a good monitor of intervention is decreased in a time-dependent manner after 1 and 3 h in warm and cold ischaemia cases. In contrast, the enthalpy increased above the control value after 6-h warm or cold tissue damage. These results confirmed that both warm and cold ischaemic injuries are detectable by deconvolution of DSC curves in the muscular intestinal layers.