Characteristics of Cymbopogon nardus L. extract with variations in leaf withering time

The focus of this study was to evaluate the effect of variation level of withering leaf on the bioactive compounds of fragrant lemongrass (Cymbopogon Nardus L.) as antioxidant and antibacterial agents. The study was conducted with 4 levels of leaf withering times, namely: fresh, 24, 48, and 72 hours. The observed parameters were colour, pH, phytochemical content, free radical trapping capacity (DPPH) and antibacterial activity. Antibacterial tests were done against Staphylococcus aureus and Escherichia coli. The data obtained were analyzed using analysis of variance (ANOVA) and further test DMRT (Duncan Multiple Range Test). The phytochemical content of the extracts from all withering times were as follows: tannins, alkaloid, flavonoid, and steroid. The best results from this study were the 2 days of withering time which have value for pH (4), color L (52), a* (60), b* (-14), and antioxidant activity (44.37%). The extract had antibacterial activity, showed by inhibitory zone 11.53±2.09 mm for S aureus and 20.35±1.13 mm against to E Coli. MIC 250-500 µg/mL and MKC 300-600 µg/mL.