Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells
This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ul...
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| Veröffentlicht in: | Food & function 2023-01, Vol.14 (2), p.978-989 |
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| creator | Zhang, Hongru Qi, Liwei Wang, Xiaodan Guo, Yujie Liu, Jiqian Xu, Yang Liu, Chengjiang Zhang, Chunhui Richel, Aurore |
| description | This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ultrasound time 40 min, CaCl
2
/peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 μg mg
−1
. The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 μg mL
−1
) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building.
A collagen peptide-calcium chelate was prepared by the ultrasound method for boosting the osteoblast activity. |
| doi_str_mv | 10.1039/d2fo02146c |
| format | Article |
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2
/peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 μg mg
−1
. The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 μg mL
−1
) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building.
A collagen peptide-calcium chelate was prepared by the ultrasound method for boosting the osteoblast activity.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/d2fo02146c</identifier><identifier>PMID: 36541828</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animals ; Biological activity ; Calcium - metabolism ; Calcium absorption ; Calcium chloride ; Calcium-sensing receptors ; Cattle ; Cell cycle ; Chelates ; Chelating Agents - chemistry ; Chelation ; Collagen ; Collagen - metabolism ; Fourier transforms ; Infrared spectroscopy ; Mineralization ; Minerals - metabolism ; Nitrogen atoms ; Osteoblasts ; Peptides ; Peptides - metabolism ; Stability analysis ; Structural analysis ; Structural stability ; Surface stability ; Thermal environments ; Ultrasonic imaging ; Ultrasonic methods ; Ultrasonic testing ; Ultrasound</subject><ispartof>Food & function, 2023-01, Vol.14 (2), p.978-989</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c267t-b4e79469f0b07016355e5e22a2be226acf2500266f5d59f5692b4d6d9e0741eb3</citedby><cites>FETCH-LOGICAL-c267t-b4e79469f0b07016355e5e22a2be226acf2500266f5d59f5692b4d6d9e0741eb3</cites><orcidid>0000-0002-4352-6542 ; 0000-0003-1959-3750 ; 0000-0002-9077-5277 ; 0000-0002-1411-4047</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36541828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hongru</creatorcontrib><creatorcontrib>Qi, Liwei</creatorcontrib><creatorcontrib>Wang, Xiaodan</creatorcontrib><creatorcontrib>Guo, Yujie</creatorcontrib><creatorcontrib>Liu, Jiqian</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Liu, Chengjiang</creatorcontrib><creatorcontrib>Zhang, Chunhui</creatorcontrib><creatorcontrib>Richel, Aurore</creatorcontrib><title>Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ultrasound time 40 min, CaCl
2
/peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 μg mg
−1
. The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 μg mL
−1
) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building.
A collagen peptide-calcium chelate was prepared by the ultrasound method for boosting the osteoblast activity.</description><subject>Animals</subject><subject>Biological activity</subject><subject>Calcium - metabolism</subject><subject>Calcium absorption</subject><subject>Calcium chloride</subject><subject>Calcium-sensing receptors</subject><subject>Cattle</subject><subject>Cell cycle</subject><subject>Chelates</subject><subject>Chelating Agents - chemistry</subject><subject>Chelation</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Fourier transforms</subject><subject>Infrared spectroscopy</subject><subject>Mineralization</subject><subject>Minerals - metabolism</subject><subject>Nitrogen atoms</subject><subject>Osteoblasts</subject><subject>Peptides</subject><subject>Peptides - metabolism</subject><subject>Stability analysis</subject><subject>Structural analysis</subject><subject>Structural stability</subject><subject>Surface stability</subject><subject>Thermal environments</subject><subject>Ultrasonic imaging</subject><subject>Ultrasonic methods</subject><subject>Ultrasonic testing</subject><subject>Ultrasound</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkk1rFTEUhgex2FK7ca8E3Ih0NMkkmclSblsVKnVRwd2QZM54UzKTMR-F23_Vf2juvW2FZpEceJ_zkbypqjcEfyK4kZ8HOnpMCRPmRXVEMaO14Pj3y8eYSXFYncR4g8tqpOxk96o6bARnpKPdUXX_M8CigkrWz8iPSCGjUnKAtJ8BGe-c-gMzWmBJdoDaKGdsnpBZg1OpUBuU1oCyS0FFn-cBTZDWfkCqhDZFFFPIJuWgXMkpfUyCYO927U6LqLR1Nm0Krtwm2ni6S9TWF9DebpUy1o9Vc93U5wQZcC6-rg5G5SKcPJzH1a-L8-vVt_ry6uv31ZfL2lDRplozaCUTcsQat5iIhnPgQKmiuuxCmZFyjKkQIx-4HLmQVLNBDBJwywjo5rj6sK-7BP83Q0z9ZON2AjWDz7GnLRdCyI7Rgr5_ht74HMqVtpToSFvenRfq454ywccYYOyXYCcVNj3B_dbL_oxeXO28XBX43UPJrCcYntBH5wrwdg-EaJ7U_5-h-QduiaUw</recordid><startdate>20230123</startdate><enddate>20230123</enddate><creator>Zhang, Hongru</creator><creator>Qi, Liwei</creator><creator>Wang, Xiaodan</creator><creator>Guo, Yujie</creator><creator>Liu, Jiqian</creator><creator>Xu, Yang</creator><creator>Liu, Chengjiang</creator><creator>Zhang, Chunhui</creator><creator>Richel, Aurore</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4352-6542</orcidid><orcidid>https://orcid.org/0000-0003-1959-3750</orcidid><orcidid>https://orcid.org/0000-0002-9077-5277</orcidid><orcidid>https://orcid.org/0000-0002-1411-4047</orcidid></search><sort><creationdate>20230123</creationdate><title>Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells</title><author>Zhang, Hongru ; Qi, Liwei ; Wang, Xiaodan ; Guo, Yujie ; Liu, Jiqian ; Xu, Yang ; Liu, Chengjiang ; Zhang, Chunhui ; Richel, Aurore</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c267t-b4e79469f0b07016355e5e22a2be226acf2500266f5d59f5692b4d6d9e0741eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Biological activity</topic><topic>Calcium - metabolism</topic><topic>Calcium absorption</topic><topic>Calcium chloride</topic><topic>Calcium-sensing receptors</topic><topic>Cattle</topic><topic>Cell cycle</topic><topic>Chelates</topic><topic>Chelating Agents - chemistry</topic><topic>Chelation</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Fourier transforms</topic><topic>Infrared spectroscopy</topic><topic>Mineralization</topic><topic>Minerals - metabolism</topic><topic>Nitrogen atoms</topic><topic>Osteoblasts</topic><topic>Peptides</topic><topic>Peptides - metabolism</topic><topic>Stability analysis</topic><topic>Structural analysis</topic><topic>Structural stability</topic><topic>Surface stability</topic><topic>Thermal environments</topic><topic>Ultrasonic imaging</topic><topic>Ultrasonic methods</topic><topic>Ultrasonic testing</topic><topic>Ultrasound</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hongru</creatorcontrib><creatorcontrib>Qi, Liwei</creatorcontrib><creatorcontrib>Wang, Xiaodan</creatorcontrib><creatorcontrib>Guo, Yujie</creatorcontrib><creatorcontrib>Liu, Jiqian</creatorcontrib><creatorcontrib>Xu, Yang</creatorcontrib><creatorcontrib>Liu, Chengjiang</creatorcontrib><creatorcontrib>Zhang, Chunhui</creatorcontrib><creatorcontrib>Richel, Aurore</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hongru</au><au>Qi, Liwei</au><au>Wang, Xiaodan</au><au>Guo, Yujie</au><au>Liu, Jiqian</au><au>Xu, Yang</au><au>Liu, Chengjiang</au><au>Zhang, Chunhui</au><au>Richel, Aurore</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2023-01-23</date><risdate>2023</risdate><volume>14</volume><issue>2</issue><spage>978</spage><epage>989</epage><pages>978-989</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ultrasound time 40 min, CaCl
2
/peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 μg mg
−1
. The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 μg mL
−1
) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building.
A collagen peptide-calcium chelate was prepared by the ultrasound method for boosting the osteoblast activity.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>36541828</pmid><doi>10.1039/d2fo02146c</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-4352-6542</orcidid><orcidid>https://orcid.org/0000-0003-1959-3750</orcidid><orcidid>https://orcid.org/0000-0002-9077-5277</orcidid><orcidid>https://orcid.org/0000-0002-1411-4047</orcidid></addata></record> |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | Animals Biological activity Calcium - metabolism Calcium absorption Calcium chloride Calcium-sensing receptors Cattle Cell cycle Chelates Chelating Agents - chemistry Chelation Collagen Collagen - metabolism Fourier transforms Infrared spectroscopy Mineralization Minerals - metabolism Nitrogen atoms Osteoblasts Peptides Peptides - metabolism Stability analysis Structural analysis Structural stability Surface stability Thermal environments Ultrasonic imaging Ultrasonic methods Ultrasonic testing Ultrasound |
| title | Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells |
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