Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ul...

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Veröffentlicht in:Food & function 2023-01, Vol.14 (2), p.978-989
Hauptverfasser: Zhang, Hongru, Qi, Liwei, Wang, Xiaodan, Guo, Yujie, Liu, Jiqian, Xu, Yang, Liu, Chengjiang, Zhang, Chunhui, Richel, Aurore
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Sprache:eng
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Zusammenfassung:This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ultrasound time 40 min, CaCl 2 /peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 μg mg −1 . The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 μg mL −1 ) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building. A collagen peptide-calcium chelate was prepared by the ultrasound method for boosting the osteoblast activity.
ISSN:2042-6496
2042-650X
DOI:10.1039/d2fo02146c