Identification of Simplicillium lanosoniveum at suppressive soil potential areas in Brantas watershed – Indonesia

Simplicillium lanosoniveum strain CG888 (MT 081944.1) is one of the fungi was isolated from the results of rhizosphere soil exploration in Gondang Manis Jamaica apple (Syzygium malaccense L), Perak District, Jombang Regency, Indonesia, which is included in the Brantas watershed area. Sampling was conducted in July 2020, using a one diagonal sampling method with five sample points. Purification of fungal isolates from soil samples was carried out using the dilution method, up to a level of 10−2. The DNA extraction results were amplified using the PCR technique. ITS4 and ITS5 primers were used for the amplification of the ITS rDNA region. The sequencing results were analyzed based on GenBank data at the National Center for Biotechnology International (NCBI). using the Basic Local Alignment Tools (BLAST) program. The phylogenic structure was determined using MEGA 7.0 software. Based on macroscopic, it has white colonies on the upper surface, and the lower is yellowish. Yellowish color is found in the old colonies or the center of the colony, and sphere. The structure is like cotton with a thick mycelia arrangement. Microscopically, The hyphae has transparent and septate, branching, tapering to the tip, and at the end formed phialid. The conidia are transparent, small and in round shape. Simplicillium lanosoniveum strain CG888 (MT 081944.1) as one of the antagonist fungi among other fungi that have been found and identified, so that it can support soil in the Brantas watershed area has the potential as a suppressive soil.