Characterization and identification of fungicide insensitive Pestalotiopsis-like species pathogenic to tea crop in India
Gray blight, a fungal disease caused by Pestalotiopsis -like species, is a widespread disease affecting tea crop ( Camellia sinensis (L.) Kuntze) in many tea-growing countries, including India, resulting in huge losses in tea production. In India, several studies have been conducted to understand th...
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Veröffentlicht in: | World journal of microbiology & biotechnology 2023-01, Vol.39 (1), p.34, Article 34 |
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Zusammenfassung: | Gray blight, a fungal disease caused by
Pestalotiopsis
-like species, is a widespread disease affecting tea crop (
Camellia sinensis
(L.) Kuntze) in many tea-growing countries, including India, resulting in huge losses in tea production. In India, several studies have been conducted to understand the fungal diseases of tea crop, but gray blight has not been well described in major tea growing areas such as in North Bengal, based on its geographic distribution, molecular analysis, or pathogenicity, and even fungicide resistance. The objective of this study was to identify and characterize the causative agents of gray blight disease in symptomatic leaf sample of tea crop collected from 27 tea gardens located in North Bengal, India and to evaluate some common fungicides against them in order to understand the resistance mechanism. In this study, we characterized
Pestalotiopsis
-like species based on the phylogenies of DNA sequences (internal transcribed spacers) and assessment of conidial characteristics. The study revealed that out of 27 isolates of gray blight pathogens, 17 belonged to the genus
Pseudopestalotiopsis (Ps.),
six isolates were
Neopestalotiopsis,
and four were
Pestalotiopsis.
Two novel species
, Ps. thailandica
and
N. natalensis
were introduced through this study
.
The most frequently isolated genus from
C. chinensis
was
Pseudopestalotiopsis.
Pathogenicity tests showed that the isolates displayed significantly different virulence when inoculated onto wounded tea leaves and the mycelial growth rate was positively correlated with pathogenicity (
P
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ISSN: | 0959-3993 1573-0972 |
DOI: | 10.1007/s11274-022-03474-3 |