Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation
The spectrophotometric method is used to count the number of microalgal cells. Regression equations were obtained for four species of the genus Tetraselmis (Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787,...
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| Veröffentlicht in: | Russian journal of marine biology 2022-12, Vol.48 (6), p.525-528 |
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| container_title | Russian journal of marine biology |
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| creator | Markina, Zh. V. Maslennikov, S. I. Botsun, L. A. |
| description | The spectrophotometric method is used to count the number of microalgal cells. Regression equations were obtained for four species of the genus
Tetraselmis
(Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787, and 0.916 for
Tetraselmis viridis
,
T. striata
,
T. maculata
, and
Tetraselmis
sp. respectively, which demonstrates the precision of this method for cell counting. |
| doi_str_mv | 10.1134/S1063074022060098 |
| format | Article |
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Tetraselmis
(Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787, and 0.916 for
Tetraselmis viridis
,
T. striata
,
T. maculata
, and
Tetraselmis
sp. respectively, which demonstrates the precision of this method for cell counting.</description><identifier>ISSN: 1063-0740</identifier><identifier>EISSN: 1608-3377</identifier><identifier>DOI: 10.1134/S1063074022060098</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Algae ; Aquatic microorganisms ; Biomedical and Life Sciences ; Biotechnology ; Cell number ; Chlorophyta ; Coefficients ; Correlation coefficient ; Freshwater & Marine Ecology ; Life Sciences ; Methods ; Microalgae ; Phytoplankton ; Short Communications ; Spectrophotometry ; Tetraselmis</subject><ispartof>Russian journal of marine biology, 2022-12, Vol.48 (6), p.525-528</ispartof><rights>Pleiades Publishing, Ltd. 2022. ISSN 1063-0740, Russian Journal of Marine Biology, 2022, Vol. 48, No. 6, pp. 525–528. © Pleiades Publishing, Ltd., 2022. Russian Text © The Author(s), 2022, published in Biologiya Morya.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c246t-2dbe2326cd33bef5afe9e92b6eef8358f5199fe6652116f297d4c6f38105fceb3</citedby><cites>FETCH-LOGICAL-c246t-2dbe2326cd33bef5afe9e92b6eef8358f5199fe6652116f297d4c6f38105fceb3</cites><orcidid>0000-0002-2098-3951 ; 0000-0001-7135-1375 ; 0000-0003-4776-0624</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1063074022060098$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1063074022060098$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Markina, Zh. V.</creatorcontrib><creatorcontrib>Maslennikov, S. I.</creatorcontrib><creatorcontrib>Botsun, L. A.</creatorcontrib><title>Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation</title><title>Russian journal of marine biology</title><addtitle>Russ J Mar Biol</addtitle><description>The spectrophotometric method is used to count the number of microalgal cells. Regression equations were obtained for four species of the genus
Tetraselmis
(Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787, and 0.916 for
Tetraselmis viridis
,
T. striata
,
T. maculata
, and
Tetraselmis
sp. respectively, which demonstrates the precision of this method for cell counting.</description><subject>Algae</subject><subject>Aquatic microorganisms</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cell number</subject><subject>Chlorophyta</subject><subject>Coefficients</subject><subject>Correlation coefficient</subject><subject>Freshwater & Marine Ecology</subject><subject>Life Sciences</subject><subject>Methods</subject><subject>Microalgae</subject><subject>Phytoplankton</subject><subject>Short Communications</subject><subject>Spectrophotometry</subject><subject>Tetraselmis</subject><issn>1063-0740</issn><issn>1608-3377</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kUtLxDAUhYso-PwB7gJudFHNo01bd1Kf4OhidF3S9MaJpE1NUsGf5r8zMyOIiKuEc79zDpebJIcEnxLCsrM5wZzhIsOUYo5xVW4kO4TjMmWsKDbjP47T5Xw72fX-FWOCMc92ks-LcTRaiqDtgKxCYQFoPoIMzo4LG2wPwWmJZhAWtkPKOnQJAVyvh1-WGoxBD1PfgvNLbaals8K8CEB6WBE3MEwePcU44cH02qPjemHssuYjiJNzVAujW7dOrSf3Dh6JoUNXb9NK86vyCMnJrIT9ZEsJ4-Hg-91Lnq-vnurb9P7x5q6-uE8lzXhIadcCZZTLjrEWVC4UVFDRlgOokuWlyklVKeA8p4RwRauiyyRXrCQ4VxJatpccrXNHZ98m8KF5tZMbYmVDi7wqSAzII0XWVNzbeweqGZ3uhftoCG6WF2r-XCh66NrjIzu8gPtJ_t_0Ben-lnU</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Markina, Zh. V.</creator><creator>Maslennikov, S. I.</creator><creator>Botsun, L. A.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><orcidid>https://orcid.org/0000-0002-2098-3951</orcidid><orcidid>https://orcid.org/0000-0001-7135-1375</orcidid><orcidid>https://orcid.org/0000-0003-4776-0624</orcidid></search><sort><creationdate>20221201</creationdate><title>Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation</title><author>Markina, Zh. V. ; Maslennikov, S. I. ; Botsun, L. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c246t-2dbe2326cd33bef5afe9e92b6eef8358f5199fe6652116f297d4c6f38105fceb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Algae</topic><topic>Aquatic microorganisms</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cell number</topic><topic>Chlorophyta</topic><topic>Coefficients</topic><topic>Correlation coefficient</topic><topic>Freshwater & Marine Ecology</topic><topic>Life Sciences</topic><topic>Methods</topic><topic>Microalgae</topic><topic>Phytoplankton</topic><topic>Short Communications</topic><topic>Spectrophotometry</topic><topic>Tetraselmis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Markina, Zh. V.</creatorcontrib><creatorcontrib>Maslennikov, S. I.</creatorcontrib><creatorcontrib>Botsun, L. A.</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Russian journal of marine biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Markina, Zh. V.</au><au>Maslennikov, S. I.</au><au>Botsun, L. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation</atitle><jtitle>Russian journal of marine biology</jtitle><stitle>Russ J Mar Biol</stitle><date>2022-12-01</date><risdate>2022</risdate><volume>48</volume><issue>6</issue><spage>525</spage><epage>528</epage><pages>525-528</pages><issn>1063-0740</issn><eissn>1608-3377</eissn><abstract>The spectrophotometric method is used to count the number of microalgal cells. Regression equations were obtained for four species of the genus
Tetraselmis
(Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787, and 0.916 for
Tetraselmis viridis
,
T. striata
,
T. maculata
, and
Tetraselmis
sp. respectively, which demonstrates the precision of this method for cell counting.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1063074022060098</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-2098-3951</orcidid><orcidid>https://orcid.org/0000-0001-7135-1375</orcidid><orcidid>https://orcid.org/0000-0003-4776-0624</orcidid></addata></record> |
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| ispartof | Russian journal of marine biology, 2022-12, Vol.48 (6), p.525-528 |
| issn | 1063-0740 1608-3377 |
| language | eng |
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| source | SpringerLink Journals (MCLS) |
| subjects | Algae Aquatic microorganisms Biomedical and Life Sciences Biotechnology Cell number Chlorophyta Coefficients Correlation coefficient Freshwater & Marine Ecology Life Sciences Methods Microalgae Phytoplankton Short Communications Spectrophotometry Tetraselmis |
| title | Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation |
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