Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation

Application of the Spectrophotometric Method for Determination of the Cell Numbers of Microalgae in the Genus Tetraselmis (Chlorophyta): Calibration Curves and Equations for Calculation

The spectrophotometric method is used to count the number of microalgal cells. Regression equations were obtained for four species of the genus Tetraselmis (Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787,...

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Veröffentlicht in:Russian journal of marine biology 2022-12, Vol.48 (6), p.525-528
Hauptverfasser: Markina, Zh. V., Maslennikov, S. I., Botsun, L. A.
Format: Artikel
Sprache:eng
Schlagworte:
Algae
Aquatic microorganisms
Biomedical and Life Sciences
Biotechnology
Cell number
Chlorophyta
Coefficients
Correlation coefficient
Freshwater & Marine Ecology
Life Sciences
Methods
Microalgae
Phytoplankton
Short Communications
Spectrophotometry
Tetraselmis
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Beschreibung
Zusammenfassung:The spectrophotometric method is used to count the number of microalgal cells. Regression equations were obtained for four species of the genus Tetraselmis (Chlorophyta), that are widely used in biotechnology. The correlation coefficients between cell number and absorbance were 0.856, 0.941, 0.787, and 0.916 for Tetraselmis viridis , T. striata , T. maculata , and Tetraselmis sp. respectively, which demonstrates the precision of this method for cell counting.
ISSN:1063-0740
1608-3377
DOI:10.1134/S1063074022060098