Type of CIDR but not eCG administration affected pregnancy per AI in Holstein heifers subjected to a 5-day estrus synchronization protocol

This study compared the effects of CIDR type and eCG treatment on ovarian follicular dynamics, estrus response and pregnancy per AI (P/AI) in Holstein heifers subjected to a 5-d estrus synchronization protocol. On Day 0, heifers (n = 133) received either a new or a once-used CIDR. On Day 5, CIDR was removed, 500 μg cloprostenol administered and heifers received or not 300 IU eCG (2 × 2 design). Heifers in estrus were inseminated 12 h after first observation. All heifers without estrus by 84 h after CIDR removal received 100 μg GnRH concurrently with AI. Ultrasonography was done in a subset of 77 heifers at CIDR insertion and removal, at AI and every 12 h after AI to determine follicular dynamics. Diameter of largest follicle at CIDR removal and AI was larger in heifers given a used CIDR (P