The Role of Prebiotics for Diversity of Intestinal Microorganisms of Tilapia (Orechromis niloticus)

Prebiotics are undigested food ingredients that can be utilized by intestinal microorganisms. The provision of prebiotics will improve growth performance, inhibit the growth of pathogens, and increase fish immunity. Several previous aquaculture studies have used single prebiotics as prebiotics, such as FOS, GOS, and inulin. We need a material that is multi prebiotics which is expected to give better results. Honey is a material that includes multi prebiotics. The purpose of this study was to evaluate the administration of prebiotic honey with different doses (0%, 05%, and 1% doses) through feeding on the diversity of microbiota in the digestive tract of tilapia ( Oreochromis niloticus ). The method used in this study was to take samples of tilapia intestines, then analyzed them using Next Generation Sequencing (NGS). The results showed that the most unique OTU were in the treatment of prebiotic honey with a dose of 1%. The treatment of prebiotic honey in the feed gave a higher OTU compared to the control. This is because the oligosaccharide content of honey can be utilized by the gut microbiota. The high unique OTUs in the treatment of adding honey, both 0.5% and 1%, presumably because these unique OTUs can grow well in that environment so that there is an equilibrium between species that together can utilize nutrients from honey oligosaccharides. so that all components of the microbiota can grow optimally. Prebiotic honey given to tilapia also triggers the emergence of the genus Lactobacillus, Cetobacterium , and Clostridium sensu stricto which are probiotic bacteria in the fisheries sector.